Raising the bar for biomolecular modeling
June 14, 2010 =The paper published in PNAS explores the role of water = molecules=20 (red and white) in forming a bridge (yellow) between proteins (green and = blue)=20 and that certain protein residues (beige) form a breakwater help the = process.=20 Credit: Aurelien de la Lande
Researchers at the University of Calgary = found that=20 amino acid residues form a type of barrier to help in the process of = electron=20 transfer between proteins.
="This raises the bar for biomolecular modeling," says Dennis Salahub, =
U of C=20
co-author of a paper published today in the prestigious journal
Electron transfer between proteins is the cornerstone of biological =
energy=20
transfer. Every life-form uses this process to convert food or sunlight =
into
The interdisciplinary team of researchers found that the electron = travels=20 over a bridge made of a water molecule, while residues on one of the = proteins=20 form a sort of 'molecular breakwater' to keep other water molecules away = while=20 the electron travels across the bridge.
"You don't want too many (water molecules around the bridge) because = it gets=20 too crowded and they're all bumping into each other and you can't get = one to fit=20 at just the right position and the right angle (for the bridge) for any = length=20 of time," says PhD student and co-author Nathan Babcock. "It's like = being on a=20 crowded subway where you can't get comfortable."
In artificial mutations with a faulty breakwater, the water bridge is = disrupted and the rate of electron transfer is markedly reduced, he = says.
Using the CHARMM molecular=20 simulation computer program, the research team examined a 40 = nanosecond=20 period of electronic coupling of the proteins methylamine dehydrogenase = and=20 amicyanin from the bacterium Paracoccus denitrificans.
"This is fundamental research but you can imagine how studies like = this can=20 be applied to various genetically modified organisms, and if you can = gain=20 control over some, you can use it to either speed up or slow down a = particular=20 reaction," says Salahub.
He says the work was made possible with the collaboration of two of = the U of=20 C's interdisciplinary research institutes; the Institute for = Biocomplexity and=20 Informatics (IBI) and the Institute for Quantum Information Science = (IQIS).
Babcock, whose background is in quantum information theory, was = pleased to do=20 research at the union of these two disciplines.
"When you think of quantum mechanics, usually you're thinking solid =
state=20
semi conductors, atoms trapped with lasers, etc. It's usually cold =
laboratory=20
stuff, not warm globby biological stuff," says the PhD student. "I think =
the=20
union of biology and quantum mechanics is very, very =
exciting."
More information: "Surface residues dynamically organize water =
bridges=20
to enhance electron transfer between proteins" was published on June 14 =
in the=20
journal Proceedings of the National Academy of Sciences (PNAS) by Nathan =
Babcock=20
et al.
Provided by University of Calgary (
=
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- Rank: 1 / 5 = (2)=20
"I think the union of biology and quantum mechanics is = very,=20 very exciting."
Very exciting indeed, I have to = agree.=20 Especially seeing that photosynthesis and the sense of smell have = already got=20 strong links to quantum mechanisms. All at room temperature. = Especially=20 exciting is the quantum entanglement that's been proposed as the = active=20 mechanism in photosynthesis.
We're in for an exciting=20 ride...!
June 14, 2010
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imgPreloader.onload=3Dfunction(){}; // clear onLoad, as IE will flip =
out w/animated gifs
return false;
}
imgPreloader.src =3D loadingImage;
// create lightbox div, same note about styles as above
var objLightbox =3D document.createElement("div");
objLightbox.setAttribute('id','lightbox');
objLightbox.style.display =3D 'none';
objLightbox.style.position =3D 'absolute';
objLightbox.style.zIndex =3D '100';=09
objBody.insertBefore(objLightbox, objOverlay.nextSibling);
=09
// create link
var objLink =3D document.createElement("a");
objLink.setAttribute('href','#');
objLink.setAttribute('title','Click to close');
objLink.onclick =3D function () {hideLightbox(); return false;}
objLightbox.appendChild(objLink);
// preload and create close button image
var imgPreloadCloseButton =3D new Image();
// if close button image found,=20
imgPreloadCloseButton.onload=3Dfunction(){
var objCloseButton =3D document.createElement("img");
objCloseButton.src =3D closeButton;
objCloseButton.setAttribute('id','closeButton');
objCloseButton.style.position =3D 'absolute';
objCloseButton.style.zIndex =3D '200';
objLink.appendChild(objCloseButton);
return false;
}
imgPreloadCloseButton.src =3D closeButton;
// create image
var objImage =3D document.createElement("img");
objImage.setAttribute('id','lightboxImage');
objLink.appendChild(objImage);
=09
// create details div, a container for the caption and keyboard message
var objLightboxDetails =3D document.createElement("div");
objLightboxDetails.setAttribute('id','lightboxDetails');
objLightbox.appendChild(objLightboxDetails);
// create caption
var objCaption =3D document.createElement("div");
objCaption.setAttribute('id','lightboxCaption');
objCaption.style.display =3D 'none';
objLightboxDetails.appendChild(objCaption);
// create keyboard message
var objKeyboardMsg =3D document.createElement("div");
objKeyboardMsg.setAttribute('id','keyboardMsg');
objKeyboardMsg.innerHTML =3D 'press esc to close';
objLightboxDetails.appendChild(objKeyboardMsg);
}
// show msg text
function ShowAlert(msg){
// prep objects
var objOverlay =3D document.getElementById('overlay');
var objLightbox =3D document.getElementById('lightbox');
var objCaption =3D document.getElementById('lightboxCaption');
var objImage =3D document.getElementById('lightboxImage');
var objLoadingImage =3D document.getElementById('loadingImage');
var objLightboxDetails =3D document.getElementById('lightboxDetails');
var arrayPageSize =3D getPageSize();
var arrayPageScroll =3D getPageScroll();
if (objOverlay) {
// set height of Overlay to take up whole page and show
objOverlay.style.height =3D (arrayPageSize[1] + 'px');
objOverlay.style.display =3D 'block';=09
// hide any previously loaded images
//objImage.src =3D '/img/warning.png';
objImage.src =3D '/img/dotted.gif';
// center lightbox and make sure that the top and left values are not =
negative
var lightboxTop =3D arrayPageScroll[1] + ( arrayPageSize[3]/2 - 100 );
var lightboxLeft =3D (arrayPageSize[0]/2 - 250);
=09
objLightbox.style.top =3D (lightboxTop < 0) ? "0px" : lightboxTop + =
"px";
objLightbox.style.left =3D (lightboxLeft < 0) ? "0px" : lightboxLeft + =
"px";
=09
objLightbox.style.width =3D '500px';
objCaption.innerHTML =3D '
//
//
//
// ' + msg + '
';
=09
objLightbox.style.display =3D 'block';
objCaption.style.display =3D 'block';
pause(500);
new Effect.Highlight('lightbox', { startcolor: '#FFFF66', endcolor: =
'#FFFFFF', restorecolor: '#FFFFFF' });
=09
// Check for 'x' keypress
Event.observe(document, 'keypress', function(event){ if(event.keyCode =
=3D=3D Event.KEY_ESC) hideLightbox();});
}
else {
if (ShowAlert.arguments[1]) alert(ShowAlert.arguments[1]);
else alert(msg);
}
return;
} =20
=20
=20
=20
function addLoadEvent_v2(func)
{=09
var oldonload =3D window.onload;
if (typeof window.onload !=3D 'function'){
window.onload =3D func;
} else {
window.onload =3D function(){
oldonload();
func();
}
}
}=09
addLoadEvent_v2(iniToolTip); // run iniToolTip onLoad
addLoadEvent_v2(initLightbox); // lighbox
------=_NextPart_000_0000_01CB0E1F.0A1F7580
Content-Type: application/octet-stream
Content-Transfer-Encoding: quoted-printable
Content-Location: http://api.toptenreviews.com/widgets/tmn-partner.js
// JavaScript Document
function detectBrowser(ie8affiliateid){
if(!(readCookie('ie8popper'))){
var currentUrl=3Dwindow.location.href;
if(!userexpiredate) {
var userexpiredate=3D1;
}
createCookie('ie8popper','read',userexpiredate);
if (/MSIE (\d+\.\d+);/.test(navigator.userAgent)){ //test for MSIE =
x.x;
var ieversion=3Dnew Number(RegExp.$1) // capture x.x portion and =
store as a number
if (ieversion<8){
document.getElementById('ie8widgetcall').innerHTML=3D"![3D"close](3D"http://www.toptenreviews.com/i/community/w_close.gif")
Close![](3D"/img/updating.gif")
';=20
$('bottom-vote').innerHTML =3D '';=20
},
onSuccess: updatevotes });
}
function updatevotes(xmlDoc){
var rank, votes;
response =3D xmlDoc.responseXML.documentElement;
if (response.getElementsByTagName('code')[0].firstChild.data =3D=3D =
200 )
{
rank =3D response.getElementsByTagName('rank')[0].firstChild.data;
votes =3D response.getElementsByTagName('total')[0].firstChild.data;
$('rank').innerHTML =3D rank +" /5 ("+ votes + " votes)";
$('bottom-vote').innerHTML =3D rank +" /5 ("+ votes + " votes)";
$('nwvoteTp').remove();
$('nwvoteBt').remove();
}
else ShowAlert("Sorry, your request can't be processed.");
=20
return;
}
=20
function moveObject(n1, e) {=20
var posX, posY; =20
var Xoffset =3D 300; =20
var Yoffset =3D -50;
if(!e)e=3Dself.event;=20
=20
if (ns4) {=20
posY =3D window.pageYOffset + e.pageY + Yoffset;
document.n1.top =3D posY;=20
}=20
else if (ie4) {=20
posY =3D document.documentElement.scrollTop + e.clientY + Yoffset; ;
document.all[n1].style.top =3D posY;=20
}=20
else if (nn6) {=20
posY =3D e.pageY + Yoffset ;
document.getElementById(n1).style.top =3D posY +"px";
}=20
}
function ShowHint(msg){
var element =3D $('iconHint');
element.innerHTML =3D msg;
element.setStyle({display: 'block'});
}
function HideHint(){
$('iconHint').hide();
}
function addStar(newsid){
var req =3D '/addStar/?newsid=3D'+newsid;
ajax =3D new Ajax.Request(req, {method: 'get', onSuccess: updateStar =
});
}
function updateStar(xmlDoc){
var msgHtml =3D 'Oops you\'re not logged in.' + 'If you are not registered, please do so, it takes = less than 60-seconds. ' + 'If you are already registered, please login here.'; =09 var msg =3D 'Oops you\'re not logged in. If you are not registered, = please do so, it takes less than 60-seconds. If you are already = registered, please login.'; =20 response =3D xmlDoc.responseXML.documentElement; if (response.getElementsByTagName('code')[0].firstChild.data =3D=3D = 200 ) $('favImg').src =3D s_imgStar; else if (response.getElementsByTagName('code')[0].firstChild.data = =3D=3D 500 ) ShowAlert(msgHtml, msg); else ShowAlert('Sorry, your request can\'t be processed.'); } function submitFeedback(){ for (var i=3D0; i < document.storyFeedback.clear.length;i++){=20 if (document.storyFeedback.clear[i].checked) var clear =3D = document.storyFeedback.clear[i].value; if (document.storyFeedback.detail[i].checked) var detail =3D = document.storyFeedback.detail[i].value; } =09 var msg =3D document.storyFeedback.msg.value; =20 var email =3D document.storyFeedback.email.value; =20 =09 var confirm =3D 'Thank you for taking your time to send in your valued = opinion to PhysOrg.com editors.
' + 'You can be assured our editors closely monitor every feedback = sent and will take appropriate actions.' + 'Your opinions are important to us. We do not guarantee = individual replies due to extremely high volume of correspondence.'; =09 var queryString =3D 'nid=3D' + nid +'&clear=3D' + escape(clear) + = '&detail=3D' + detail +'&msg=3D' + escape(msg) + '&email=3D' + = escape(email); submit =3D new Ajax.Request('/addFeedback/', { method: 'post', parameters: queryString, onCreate: function(){ ShowAlert(confirm); } }); =20 } function createFeedback(){ ajax =3D new Ajax.Request('/addFeedback/', { method: 'get', onSuccess: function(xmlDoc){ response =3D xmlDoc.responseText; ShowAlert(response, 'Your request cannot be processed at this = moment'); } }); } function showFeeback(){ document.write('send feedback to editors'); } ------=_NextPart_000_0000_01CB0E1F.0A1F7580 Content-Type: application/octet-stream Content-Transfer-Encoding: quoted-printable Content-Location: http://www.physorg.com/js/adsense_news_page2.js function google_ad_request_done(google_ads) { var s =3D ''; var i; var goog_url =3D''; =09 if (google_ads.length =3D=3D 0) { return; } =09 s =3D '
';=20
// 'Ads by google' =09
goog_url +=3D'
' + '
';
=09
document.write(s);
return;
} // end func
------=_NextPart_000_0000_01CB0E1F.0A1F7580
Content-Type: application/octet-stream
Content-Transfer-Encoding: quoted-printable
Content-Location: http://pagead2.googlesyndication.com/pagead/show_ads.js
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l=3Dthis,ba=3Dfunction(a,b,c){a=3Da.split(".");c=3Dc||l;!(a[0]in =
c)&&c.execScript&&c.execScript("var "+a[0]);for(var =
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e c=3Dc[d]?c[d]:(c[d]=3D{})},m=3Dfunction(a){var b=3Dtypeof =
a;if(b=3D=3D"object")if(a){if(a instanceof Array||!(a instanceof =
Object)&&Object.prototype.toString.call(a)=3D=3D"[object Array]"||typeof =
a.length=3D=3D"number"&&typeof a.splice!=3D"undefined"&&typeof =
a.propertyIsEnumerable!=3D"undefined"&&!a.propertyIsEnumerable("splice"))=
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if(!(a instanceof =
Object)&&(Object.prototype.toString.call(a)=3D=3D"[object =
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c=3Db||l;if(arguments.length>2){var =
d=3DArray.prototype.slice.call(arguments,2);return function(){var =
e=3DArray.prototype.slice.call(arguments);Array.prototype.unshift.apply(e=
,d);return a.apply(c,e)}}else return function(){return =
a.apply(c,arguments)}},ea=3Dfunction(a){var =
b=3DArray.prototype.slice.call(arguments,1);return function(){var =
c=3DArray.prototype.slice.call(arguments);c.unshift.apply(c,b);return =
a.apply(this,c)}},r=3Dfunction(a,b,c){ba(a,b,c)},fa=3Dfunction(a,=0A=
b,c){a[b]=3Dc};var s=3Dfunction(a,b){var c=3DparseFloat(a);return =
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urn b;var c=3Da.match(ga);return c?c[0]:b};var ha=3Dfunction(){return =
u("","googleads.g.doubleclick.net")},ia=3Dfunction(){return =
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u("","pagead2.googlesyndication.com")};var =
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' =09 if (google_ads[0].type =3D=3D "flash") { s +=3D ''; if (google_info.feedback_url) { s +=3D 'Ads by = Google'; } else { s +=3D 'Ads by Google';}=20 s +=3D '
'; s +=3D ''; } else if (google_ads[0].type =3D=3D "image") { s +=3D ''; if (google_info.feedback_url) { s +=3D 'Ads by = Google'; } else { s +=3D 'Ads by Google';}=20 s +=3D '
'; s +=3D ''; if (google_info.feedback_url) { s +=3D 'Ads by = Google'; } else { s +=3D 'Ads by Google';}=20 s +=3D '
'; s +=3D '' + '
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'; =09 =09 } else { if (google_ads.length =3D=3D 1) { s +=3D goog_url +''+=20 '' + google_ads[0].line1 + " " + ' - ' + google_ads[0].line2 + ' ' + google_ads[0].line3 + =20 ' - '+ '' + = =20 google_ads[0].visible_url + '' + =09 '
';=09 } else if (google_ads.length > 1) {=09 s +=3D goog_url; for(i =3D 0; i < google_ads.length; ++i) { s +=3D '
' + '' + google_ads[i].line1 + " " + ' - ' + google_ads[i].line2 + ' ' + google_ads[i].line3 + ' - '+ '' + = =20 google_ads[i].visible_url + '' + '
';=09 }=20 s +=3D ''; } } =09 if (google_ads[0].bidtype =3D=3D "CPC") { google_adnum =3D = google_adnum + google_ads.length; } =09 s +=3D'
' +=0A=
'![](3D"')
'=0A=
);=0A=
this.e_base =3D get_element('sl' + this.n_id + 'base');=0A=
this.e_slider =3D get_element('sl' + this.n_id + 'slider');=0A=
=0A=
// safely hook document/window events=0A=
if (document.onmousemove !=3D f_sliderMouseMove) {=0A=
window.f_savedMouseMove =3D document.onmousemove;=0A=
document.onmousemove =3D f_sliderMouseMove;=0A=
}=0A=
if (document.onmouseup !=3D f_sliderMouseUp) {=0A=
window.f_savedMouseUp =3D document.onmouseup;=0A=
document.onmouseup =3D f_sliderMouseUp;=0A=
}=0A=
// preset to the value in the input box if available=0A=
var e_input =3D this.s_form =3D=3D null=0A=
? get_element(this.s_name)=0A=
: document.forms[this.s_form]=0A=
? document.forms[this.s_form].elements[this.s_name]=0A=
: null;=0A=
this.f_setValue(e_input && e_input.value !=3D '' ? e_input.value : =
null, 1);=0A=
this.e_slider.style.visibility =3D 'visible';=0A=
}=0A=
=0A=
function f_sliderSetValue (n_value, b_noInputCheck) {=0A=
if (n_value =3D=3D null)=0A=
n_value =3D this.n_value =3D=3D null ? this.n_minValue : this.n_value;=0A=
if (isNaN(n_value))=0A=
return false;=0A=
// round to closest multiple if step is specified=0A=
if (this.n_step)=0A=
n_value =3D Math.round((n_value - this.n_minValue) / this.n_step) * =
this.n_step + this.n_minValue;=0A=
// smooth out the result=0A=
if (n_value % 1)=0A=
n_value =3D Math.round(n_value * 1e5) / 1e5;=0A=
=0A=
if (n_value < this.n_minValue)=0A=
n_value =3D this.n_minValue;=0A=
if (n_value > this.n_maxValue)=0A=
n_value =3D this.n_maxValue;=0A=
=0A=
this.n_value =3D n_value;=0A=
=0A=
// move the slider=0A=
if (this.b_vertical)=0A=
this.e_slider.style.top =3D (this.n_pathTop + this.n_pathLength - =
Math.round((n_value - this.n_minValue) * this.n_pix2value)) + 'px';=0A=
else=0A=
this.e_slider.style.left =3D (this.n_pathLeft + Math.round((n_value - =
this.n_minValue) * this.n_pix2value)) + 'px';=0A=
=0A=
// save new value=0A=
var e_input;=0A=
if (this.s_form =3D=3D null) {=0A=
e_input =3D get_element(this.s_name);=0A=
if (!e_input)=0A=
return b_noInputCheck ? null : f_sliderError(this.n_id, "Can not find =
the input with ID=3D'" + this.s_name + "'.");=0A=
}=0A=
else {=0A=
var e_form =3D document.forms[this.s_form];=0A=
if (!e_form)=0A=
return b_noInputCheck ? null : f_sliderError(this.n_id, "Can not find =
the form with NAME=3D'" + this.s_form + "'.");=0A=
e_input =3D e_form.elements[this.s_name];=0A=
if (!e_input)=0A=
return b_noInputCheck ? null : f_sliderError(this.n_id, "Can not find =
the input with NAME=3D'" + this.s_name + "'.");=0A=
}=0A=
e_input.value =3D n_value;=0A=
}=0A=
=0A=
// get absolute position of the element in the document=0A=
function f_sliderGetPos (b_vertical, b_base) {=0A=
var n_pos =3D 0,=0A=
s_coord =3D (b_vertical ? 'Top' : 'Left');=0A=
var o_elem =3D o_elem2 =3D b_base ? this.e_base : this.e_slider;=0A=
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while (o_elem) {=0A=
n_pos +=3D o_elem["offset" + s_coord];=0A=
o_elem =3D o_elem.offsetParent;=0A=
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o_elem =3D o_elem2;=0A=
=0A=
var n_offset;=0A=
while (o_elem.tagName !=3D "BODY") {=0A=
n_offset =3D o_elem["scroll" + s_coord];=0A=
if (n_offset)=0A=
n_pos -=3D o_elem["scroll" + s_coord];=0A=
o_elem =3D o_elem.parentNode;=0A=
}=0A=
return n_pos;=0A=
}=0A=
=0A=
function f_sliderMouseDown (n_id) {=0A=
window.n_activeSliderId =3D n_id;=0A=
return false;=0A=
}=0A=
=0A=
function f_sliderMouseUp (e_event, b_watching) {=0A=
if (window.n_activeSliderId !=3D null) {=0A=
var o_slider =3D window.A_SLIDERS[window.n_activeSliderId];=0A=
o_slider.f_setValue(o_slider.n_minValue + (o_slider.b_vertical=0A=
? (o_slider.n_pathLength - parseInt(o_slider.e_slider.style.top) + =
o_slider.n_pathTop)=0A=
: (parseInt(o_slider.e_slider.style.left) - o_slider.n_pathLeft)) / =
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if (b_watching) return;=0A=
window.n_activeSliderId =3D null;=0A=
}=0A=
if (window.f_savedMouseUp)=0A=
return window.f_savedMouseUp(e_event);=0A=
}=0A=
=0A=
function f_sliderMouseMove (e_event) {=0A=
=0A=
if (!e_event && window.event) e_event =3D window.event;=0A=
=0A=
// save mouse coordinates=0A=
if (e_event) {=0A=
window.n_mouseX =3D e_event.clientX + f_scrollLeft();=0A=
window.n_mouseY =3D e_event.clientY + f_scrollTop();=0A=
}=0A=
=0A=
// check if in drag mode=0A=
if (window.n_activeSliderId !=3D null) {=0A=
var o_slider =3D window.A_SLIDERS[window.n_activeSliderId];=0A=
=0A=
var n_pxOffset;=0A=
if (o_slider.b_vertical) {=0A=
var n_sliderTop =3D window.n_mouseY - o_slider.n_sliderHeight / 2 - =
o_slider.f_getPos(1, 1) - 3;=0A=
// limit the slider movement=0A=
if (n_sliderTop < o_slider.n_pathTop)=0A=
n_sliderTop =3D o_slider.n_pathTop;=0A=
var n_pxMax =3D o_slider.n_pathTop + o_slider.n_pathLength;=0A=
if (n_sliderTop > n_pxMax)=0A=
n_sliderTop =3D n_pxMax;=0A=
o_slider.e_slider.style.top =3D n_sliderTop + 'px';=0A=
n_pxOffset =3D o_slider.n_pathLength - n_sliderTop + =
o_slider.n_pathTop;=0A=
}=0A=
else {=0A=
var n_sliderLeft =3D window.n_mouseX - o_slider.n_sliderWidth / 2 - =
o_slider.f_getPos(0, 1) - 3;=0A=
// limit the slider movement=0A=
if (n_sliderLeft < o_slider.n_pathLeft)=0A=
n_sliderLeft =3D o_slider.n_pathLeft;=0A=
var n_pxMax =3D o_slider.n_pathLeft + o_slider.n_pathLength;=0A=
if (n_sliderLeft > n_pxMax)=0A=
n_sliderLeft =3D n_pxMax;=0A=
o_slider.e_slider.style.left =3D n_sliderLeft + 'px';=0A=
n_pxOffset =3D n_sliderLeft - o_slider.n_pathLeft;=0A=
}=0A=
if (o_slider.b_watch)=0A=
f_sliderMouseUp(e_event, 1);=0A=
=0A=
return false;=0A=
}=0A=
=0A=
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Content-Type: application/octet-stream
Content-Transfer-Encoding: quoted-printable
Content-Location: http://www.physorg.com/js/comments.js
var lastCMT =3D '';
function UpdateFilter(val){
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else $('SliderFilter').innerHTML =3D ': Off';
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=20
=20
function DisplayComments(){
ajax =3D new Ajax.Updater('comments', =
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lter=3D'+fltthres, {
method: 'get',
onCreate: function() {if ( $('cmThr') ) $('cmThr').innerHTML =3D =
updIMG;},
onComplete: function() {if ( $('cmThr') ) $('cmThr').innerHTML =
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PostBox();
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function ChangeSort(){
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DisplayComments();
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var lgbox =3D '' +
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document.getElementById('postbox').innerHTML =3D pstbx;
document.commadd.nid.value =3D nid;
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document.getElementById('postbox').innerHTML =3D lgbox;
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function SignIn(formName, handler){
var email =3D formName.email.value;
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var passwd =3D formName.passwd.value;
if ( passwd.length =3D=3D 0 ){ ShowAlert('Please enter your =
password!'); return } =09
var queryString =3D 'email=3D' + escape(email) + '&passwd=3D' + passwd =
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ajax =3D new Ajax.Request('/comments/',{
method: 'post',
parameters: queryString,
onCreate: function(){$('cmPrThr').innerHTML =3D updIMG;},
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});
}=20
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function AddComment(){
var cmnt =3D document.commadd.commenttext.value;
if ( cmnt.length =3D=3D 0 ){ ShowAlert('Please enter your comment'); =
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if ( cmnt.length > 1000 ){ ShowAlert('Comment text too long'); return =
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var cid =3D document.commadd.cid.value;
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submit =3D new Ajax.Request('/comments/', {
method: 'post',
parameters: queryString,
onCreate: function(){
document.commadd.commentsubmit.disabled =3D true;
$('cmPrThr').innerHTML =3D updIMG;
},
onSuccess: function(respXML){
response =3D respXML.responseXML.documentElement;
if (response.getElementsByTagName('msg')[0].firstChild !=3D =
null) =
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DisplayComments();
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} =09
=20
});
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ajax =3D new Ajax.Request('/comments/?code=3DCOM&cid=3D'+cid,
{
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new Effect.ScrollTo('postbox', { duration: 0.5 });
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response =3D xmlDoc.responseXML.documentElement;
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=
ShowAlert(response.getElementsByTagName('msg')[0].firstChild.data);
return ;
}
var commentTEXT =3D =
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0].firstChild.data;
//document.getElementById('addtitle').innerHTML =3D 'Edit =
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$('addtitle').innerHTML =3D 'Edit comment';
document.commadd.cid.value =3D cid;
document.commadd.status.value =3D'edit';
document.commadd.commentsubmit.value=3D'Edit';
document.commadd.commenttext.value =3D commentTEXT;
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});
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document.commadd.commenttext.value =3D '';=20
document.commadd.status.value =3D'New';
document.commadd.cid.value =3D '0';
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document.commadd.reset;
}
function ShowComment(cid){
ajax =3D new =
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method: 'get',
onSuccess: function(xmlDoc){
response =3D xmlDoc.responseXML.documentElement;
cmTXT =3D =
response.getElementsByTagName('comment')[0].getElementsByTagName("text")[=
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$('cm_'+cid).innerHTML =3D cmTXT;
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}
});
}
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ajax =3D new Ajax.Request('/comments/?code=3DCOM&cid=3D'+cid, {
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onCreate: function() {$('cmPrThr').innerHTML =3D updIMG;},
onComplete: function() {$('cmPrThr').innerHTML =3D '';},
onSuccess: function(xmlDoc){
response =3D xmlDoc.responseXML.documentElement;
cmTXT =3D =
response.getElementsByTagName('comment')[0].getElementsByTagName("text")[=
0].firstChild.data;
if (cmTXT !=3D '') {
document.commadd.commenttext.value =3D '[q]' + cmTXT + =
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new Effect.ScrollTo('postbox', { duration: 0.5 });
}
}
});
}
=09
=09
=09
// Rank handling=20
function RateComment(cid, rank){
=09
ajax =3D new =
Ajax.Request('/comments/?code=3DRATE&cid=3D'+cid+'&rank=3D'+rank, {
method: 'get',
onCreate: function(){$('rate-' + cid).hide();
$('rk-' + cid).innerHTML =3D updIMG;=09
},
onSuccess: DisplayRank
});
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function DisplayRank(xmlDoc){
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if (response.getElementsByTagName('msg')[0].firstChild !=3D null) {
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return;
}
var cid =3D =
response.getElementsByTagName('comment')[0].getElementsByTagName('cid')[0=
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var rank=3D =
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var votes=3D =
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$('rk-' + cid).innerHTML =3D FormatRank(rank, votes);=20
=09
}
// fstlog
function SignInComments(xmlDoc){
response =3D xmlDoc.responseXML.documentElement; =09
=09
if (response.getElementsByTagName('msg')[0].firstChild !=3D null) {
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return;
} =20
if (response.getElementsByTagName('mid')[0].firstChild !=3D null) {
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}=09
DisplayComments(); =20
PostBox();
}
// flag comment
function FlagComment(cid){
var alertBox =3D 'Report this comment as spam, offensive or =
inappropriate?'+
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' + ''+
'Sign in with
' +
'' + =09
'Forgot your password? Click =
here to reset it.
' + 'Notify me via email of follow-up comments posted here ' + '' +=20 '
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